The extraction and purification of polysaccharides from date seeds (Phoenix dactilyfera L.), as well as the evaluation of their antioxidant and antiglycated activities in vitro were investigated. Aqueous and oxalate extracts (PPW and PPO) were obtained by sequential extraction from date seeds powder. Sugar contents and macromolecular characteristics were estimated by colorimetric assays and size exclusion chromatography (SEC/MALS/VD/DRI). The best yield was obtained for aqueous extract PPW (1.9%). These experiments showed that all extracted samples were polysaccharides having a moderately high molecular weight (1680 and 1200 kDa for PPW and PPO, respectively). Preliminary antioxidant and antiglycated tests indicated that PPW showed even better ability in the scavenging of DPPH• and NO• radicals and the inhibition of lipid peroxidation and protein glycation. These findings suggest that water-soluble polysaccharides from date seeds could be explored as free radical scavengers and protein glycation inhibitors.
W. Marzouk, M.A. Chaouch, J. Hafsa, D. LeCerf, H. Majdoub
Phoenix dactilyfera L., date seeds, polysaccharide, antioxidant activitiy, antiglycated activity