Mise au point d’une méthode d’analyse rapide et simple de quatre tétracyclines dans les muscles bovins par HPLC-MS
Written by sctunisie no commentsThe aim of this work is to develop and validate a multi residue analytical method for a rapid and simple determination of Oxytetracycline, Tetracycline, Chlortetracycline and Doxycycline in bovine meat by HPLC-MS. Solid phase extraction of these antibiotics was made on a C18 cartridge that was first activated with 3 mL of methanol and 6 mL of water. This mixture was then eluted with 2 mL of a hydroalcoholic solution (5 % methanol). The antibiotic mixture was adsorbed on the stationary phase of the activated cartridge and eluted twice with 2.5 mL of methanol. The chromatographic separation was obtained on a RP-C18 Omnisher type column and using water (adjusted at pH 2.7 with a 20 mM solution of TFA) and acetonitrile in a gradient elution mode (20 % for 1 min and from 20 to 100 % with acetonitrile in 8 min). The flow was maintained constant at 0.25 mL/min. Quantitative analysis of antibiotics residues was made by HPLC-MS in SIM detection mode. This method allowed the determination of the four antibiotics with good yields: 81.9 ± 2.5 % for Oxytetracycline, 89.6 ± 1.5 % for Tetracycline, 80.1 ± 1.6 % for Chlortetacyclirte and 69.6 ± 2.9 % for Doxycycline.
S. Barrek, O. Paisse, H. Zarrouk, M.F. Grenier-Loustalot
residue, antibiotics, bovine meat, HPLC-MS
Pages 195-203