JOURNAL of the Tunisian Chemical Society

The modified Stöber silica particles were used as support for the development of molecular imprinted polymer matrix for extraction of patulin. γ-MPTS was covalently attached to the surface of Stöber silica particles by means of condensation reaction between surface silanol groups of silica and methoxy groups of γ-MPTS. The prepared silica SiO₂‑γ‑MPTS was polymerized through a non-covalent approach using patulin (PAT) as a template, maleic acid (MA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross linker, 2,2-azobis- (2- methylpropionitrile) (AIBN) as a precursor and acetonitrile (MeCN) as a porogen solvent to prepare SiO₂MA@MIP. The non-imprinted polymer SiO₂MA@NIP was prepared following the same synthetic scheme, but in the absence of the template. Molecular imprinted material was used to selective solid phase extraction (SPE) of patulin in apple matrix. The best conditions for PAT extraction using the novel MIP@SPE were: 50 mg mass of SiO₂MA@MIP packing in solid phase extraction cartridges, solution of sodium bicarbonate with (1%) acetic acid as washing solvent and 5 mL MeCN as eluting solvent. The developed MIP@SPE has the advantages of MIPs and SPE and could have potential applications for high selective enrichment and determination of PAT in apple juices with a major impact on quality control in food processing, improving product quality and safety with minimal investment.

A. Anene, K. Hosni, Y. Chevalier, R. Kalfat, S. Hbaieb

Patulin, molecularly imprinted polymer, solid phase extraction, MIP@SPE cartridge

Pages 187-192