Phenolic compounds with antioxidant activity from olive mill wastewaters
Written by sctunisie no commentsIn this investigation, different methods for the extraction and the purification of phenolic compounds from the olive mill wastewaters (OMW) were used. Consequently, seven phenolic monomers were isolated at pure grade. By means of spectroscopic methods (IR, UV, MS, 1H NMR, HPLC-UV and HPLC-MS), their structures were established as tyrosol, hydroxytyrosol, caffeic acid, protocatechuic acid, para-coumaric acid, apigenin and luteolin. The antiradical activity of the purified phenolic compounds was evaluated by measuring the radical scavenging effect on 1.1-diphenyl 2-picrylhydrazyl (DPPH). Result showed that hydroxytyrosol presented the highest DPPH radical scavenging effect. The antioxidative activity of the isolated compounds was evaluated using a coupled oxidation of β-carotene-linoleic acid method. The antioxidant potency of the various tested polyphenols was in the following decreasing order: hydroxytyrosol > caffeic acid> protocatechuic acid > para-coumane acid > tyrosol.
N. Allouche, S. Sayadi, M. Damak
olive mill wastewaters, polyphenols, isolation, antioxidants, β-carotene, 1.1-diphenyl 2-picrylhydrazyl
Pages 33-43
