An empirical model for predicting a selectivity factor in HPLC
Written by sctunisie no commentsThe aim of this work is the modelisation of the selectivity factor α versus the nature of the couple of solutes and the percentage of the organic modifier, on a column (Spherisorb ODS-2, 5 µm) and with a mobile phase acetonitrile/water or methanol/water varying between 40/60 and 80/20 in vol/vol. The nature of the couple of solutes is represented by the value of the logarithm of its selectivity factor α at 60% of organic modifier. This model allow the prediction of the mobile phase composition with which separation of solutes is possible.
F. Hellal, M. Debbabi
Pages 895-906
